Hydrochloric acid-enhanced radiofrequency ablation for treating a large hepatocellular carcinoma with spontaneous rapture: a case report
© The Author(s) 2017
Received: 20 October 2015
Accepted: 10 October 2016
Published: 7 January 2017
A ruptured hepatocellular carcinoma (HCC) is often fatal. In addition to surgery and transarterial embolization, radiofrequency ablation (RFA) might be another option for treating a ruptured HCC. Unfortunately, conventional RFA has a limited ablation zone; as such, it is rarely used to treat ruptured tumors.
This case was a 60-year-old man who had a large, ruptured HCC in which hydrochloric acid (HCl)-enhanced RFA successfully controlled the bleeding and made the tumor completely necrotic.
Considering the effectiveness of HCl-enhanced RFA in achieving hemostasis and tumor ablation, it might be a new option for treating large, ruptured HCCs.
KeywordsHydrochloric acid Radiofrequency ablation Spontaneous rupture Hepatocellular carcinoma
Liver injuries are common and often result in abdominal pain, bleeding, hypotension, and other conditions requiring immediate attention. Hepatic hemorrhage most commonly occurs because of blunt trauma or the spontaneous rupture of hepatic neoplasms, including hepatocellular carcinoma (HCC), adenoma, focal nodular hyperplasia, and hemangioma.
In Asia and Africa, the most common neoplastic cause of hepatic hemorrhage is HCC, owing to the high prevalence of the disease . The occurrence rate of spontaneously ruptured HCC ranges from 3% to 14.5%; a ruptured HCC with intraperitoneal hemorrhage is a life-threatening complication with a 30-day hospital mortality between 31% and 67% [2, 3]. Several factors are associated with hemorrhagic rupture of HCC, including trauma, large tumor size, vascular invasion, multiple nodules, prior transarterial chemoembolization (TACE), portal venous hypertension, and vascular injury [3, 4]. Hepatic hemorrhage related to a ruptured HCC can rapidly cause hemodynamic instability that requires emergency treatment.
Treatment options for ruptured hemorrhagic HCC include packing, plication, emergent hepatic artery ligation, hepatectomy, transarterial embolization (TAE), and radiofrequency ablation (RFA) [3, 5, 6]. In some cases, TAE and surgery are not effective. Moreover, a 3-cm ablative zone produced by a single RFA probe or a 5-cm ablative zone obtained with a cluster of probes is not sufficient to ablate a large tumor. However, hydrochloric acid (HCl)-enhanced RFA has a much larger ablation zone, making it potentially suitable for controlling bleeding from a large, ruptured HCC.
Written informed consent to the procedure and consent to publish were obtained from the patient and his family. The HCl-enhanced RFA procedure was approved by the Clinical Research Ethics Committee of Sun Yat-sen Cancer Center.
Spontaneous tumor rupture is a potential consequence of HCC and results in a high mortality . The most common mechanisms for hemoperitoneum in HCC rupture are tumor pseudo-capsule disruption and tearing of a parasitic feeding artery. Kanematsu et al.  hypothesized that several features visible on CT images portend HCC rupture: a large tumor size, an area of protruded tumor, and a large extent of contact between the tumor and the liver surface. Consistent with this hypothesis, the large HCC in this case eventually ruptured.
Hemostasis is the primary consideration in HCC rupture, and adequate bleeding controlling is vital to patient prognosis. As treatment opinions regarding ruptured HCC have evolved and as new clinical technologies have been adopted, treatment strategies for ruptured HCC have become increasingly diverse, including various conservative, surgical, and minimally invasive measurements. In 1965, Ong et al.  advocated packing the ruptured liver as an efficient method to control hemorrhage; then, in 1972, they advocated ligating the hepatic artery. However, with simple packing, they found a high rate of re-bleeding. Moreover, in some cases in the 1970s, direct plication by overlying the capsule was determined to achieve hemostasis . Because the results were disappointing, though, given the friability of HCC, this method is now seldom used. Although emergent hepatectomy remains the most effective treatment for ruptured HCC, it accounts for 7% of the hospital mortality .
With the increasing application of interventional techniques in the last 20 years, TAE emerged as another procedure that could potentially achieve hemostasis in a ruptured HCC, having a success rate between 53% and 100% and resulting in a lower 30-day hospital mortality (0%–37%) than those of surgery . The advantages of TAE over surgery are that it is minimally invasive and directly occludes the feeding vessels, which means that surgery can be avoided in patients at high risk.
Recently, several studies reported successful hemostasis using RFA [6, 10]. In addition, Chueng et al.  found that RFA is safe and effective for achieving hemostasis in ruptured HCC with satisfactory survival outcomes, and therefore they proposed RFA as a first-line option for treating a ruptured HCC. However, needle placement with laparoscopic RFA is less accurate than that with image-guided percutaneous RFA, which has particular advantages for treating lesions within the deep hepatic parenchyma.
In the present case, because our patient had severe cirrhosis, he could not tolerate surgery. Moreover, a failed TAE impaired his hepatic function. As Leung et al.  reported, non-super-selective TAE as regional therapy is harmful to patients. They thought that there was typically only one opportunity to achieve hemostasis with TAE, and that more than one attempt would harm the normal hepatic parenchyma.
When the tumors are large or peripherally located, extrahepatic collateral arteries commonly supply HCCs . In our case, both enhanced CT scanning and DSA revealed a filling defect on the lower portion of the tumor. Thus, considering the location of the non-enhanced tumor, collateral vessels supplying this portion were probably branches of the adrenal artery.
After TAE failed to restore hemostasis, and considering both the patient’s overall condition and the results of our previous clinical studies with HCl-enhanced RFA, we determined that CT-guided RFA was a reasonable treatment option. A prior PSE had increased the patient’s platelet count to the level on which coagulation was adequate to proceed with RFA . However, currently, the maximum ablative zone obtainable with conventional monopolar RFA is 3 cm in diameter. Thus, in this case, the 7-cm diameter tumor would be difficult to ablate in one session. Additionally, our previous experiments showed that infusing diluted HCl instead of natural saline into the ablation zone during RFA with monopolar perfusion electrodes could enlarge the ablation size (mean ± standard deviation) from 3.49 ± 0.07 to 6.85 ± 0.32 cm under the same power and time settings (30 W and 30 min) . Given these considerations and the patient’s large hepatic mass, we decided to use HCl-enhanced RFA instead of natural saline-infused RFA. In our case, HCl-enhanced RFA damaged neither the nearby duodenum nor the renal fascia. This result may relate to the progressive decrease in temperature from the center to the periphery and to the insulating effects of the fibrous fascia.
In contrast to normal saline, HCl functions as a chemical ablation agent and is capable of producing an ablative lesion itself . Furthermore, HCl is a strong electrolyte whose conductivity is about three times that of natural saline, which can increase the conductivity of RF energy. Thus, when HCl is instilled around the RF electrode, the RF energy expands from a needle shape into a spherical shape. This enlarged spherical volume increases contact area between the RF source and the tissue intended for ablation. In turn, this increased contact area transfers and sustains RF energy much longer (100 min in this case) than normal saline, lengthening the charred area and prolonging the delivery of energy, thus creating a larger ablation zone than any other current RFA method. Therefore, our success in treating this ruptured HCC suggests that a treatment pattern of HCl-enhanced RFA followed by TAE or combined with surgery could be considered in the future.
As in the present case, for patients with a cirrhotic liver, HCC is often associated with survival of less than 30 days . However, our patient has survived for 16 months. Treatment with HCl-enhanced RFA achieved immediate and total hemostasis and completely ablated the large, bleeding tumor. In addition, as a regional therapy, HCl-enhanced RFA resulted in well-preserved liver function; thus, the patient could recuperate rapidly.
In conclusion, HCl-enhanced RFA appears to be a safe and effective treatment for controlling bleeding after spontaneous rupture of large HCC. However, further research is necessary to confirm our findings.
JHH, YKG, FG, WY, and YYZ were responsible for the patient’s treatment. FA, RHZ, YKG, and FG collected the patient’s information. TQZ, WY, and XYJ mainly searched the references for this manuscript.JHH, JNM, TQZ, and FA drafted this manuscript. JHH, JNM, and YKG offered constructive suggestions for this study. All authors read and approved the final manuscript.
This work was supported by grants from the National Natural Science Foundation of China (No. 81371652) and the Science and Technology Planning Project of Guangdong Province (No. 2012B031800120).
The authors declare that they have no any actual or potential competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Chen W, Zheng R, Zeng H, Zhang S. The incidence and mortality of major cancers in China, 2012. Chin J Cancer. 2016;35(1):73.View ArticlePubMedPubMed CentralGoogle Scholar
- Cheung TT, Poon RT, Chok KS, Chan AC, Tsang SH, Dai WC, et al. Management of spontaneously ruptured hepatocellular carcinomas in the radiofrequency ablation era. PLoS ONE. 2014;9(4):e94453.View ArticlePubMedPubMed CentralGoogle Scholar
- Lai EC, Lau WY. Spontaneous rupture of hepatocellular carcinoma: a systematic review. Arch Surg. 2006;141(2):191–8.View ArticlePubMedGoogle Scholar
- Liu CL, Ngan H, Lo CM, Fan ST. Ruptured hepatocellular carcinoma as a complication of transarterial oily chemoembolization. Br J Surg. 1998;85(4):512–4.View ArticlePubMedGoogle Scholar
- Kim JY, Lee JS, Oh DH, Yim YH, Lee HK. Transcatheter arterial chemoembolization confers survival benefit in patients with a spontaneously ruptured hepatocellular carcinoma. Eur J Gastroenterol Hepatol. 2012;24(6):640–5.View ArticlePubMedGoogle Scholar
- Ng KK, Lam CM, Poon RT, Law WL, Seto CL, Fan ST. Radiofrequency ablation as a salvage procedure for ruptured hepatocellular carcinoma. Hepatogastroenterology. 2003;50(53):1641–3.PubMedGoogle Scholar
- Luo RG, Fao F, Huang JH, Gu YK, Jiang XY, Huang YJ. Diluted hydrochloric acid generates larger radiofrequency ablation lesions in excised porcine livers. Diagn Interv Radiol (Ankara, Turkey). 2013;19(2):145–9.Google Scholar
- Kanematsu M, Imaeda T, Yamawaki Y, Seki M, Goto H, Sone Y, et al. Rupture of hepatocellular carcinoma: predictive value of CT findings. Am J Roentgenol. 1992;158(6):1247–50.View ArticleGoogle Scholar
- Ong GB, Taw JL. Spontaneous rupture of hepatocellular carcinoma. Br Med J. 1972;4(5833):146–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Fuchizaki U, Miyamori H, Kitagawa S, Kaneko S. Radiofrequency ablation for life-threatening ruptured hepatocellular carcinoma. J Hepatol. 2004;40(2):354–5.View ArticlePubMedGoogle Scholar
- Leung KL, Lau WY, Lai PB, Yiu RY, Meng WC, Leow CK. Spontaneous rupture of hepatocellular carcinoma: conservative management and selective intervention. Arch Surg. 1999;134(10):1103–7.View ArticlePubMedGoogle Scholar
- Kim HC, Chung JW, Lee W, Jae HJ, Park JH. Recognizing extrahepatic collateral vessels that supply hepatocellular carcinoma to avoid complications of transcatheter arterial chemoembolization. Radiographics. 2005;25(Suppl 1):S25–39.View ArticlePubMedGoogle Scholar
- Huang JH, Gao F, Gu YK, Li WQ, Lu LW. Combined treatment of hepatocellular carcinoma with partial splenic embolization and transcatheter hepatic arterial chemoembolization. World J Gastroenterol. 2007;13(48):6593–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Jiang XY, Gu YK, Huang JH, Gao F, Zou RH, Zhang TQ. Ex vivo liver experiment of hydrochloric acid-infused and saline-infused monopolar radiofrequency ablation: better outcomes in temperature, energy, and coagulation. Cardiovasc Intervent Radiol. 2016;39(4):600–5.View ArticlePubMedGoogle Scholar
- Weijian F, Zan L, Suhong H, Hongmei Z, Lei Z, Yanjie Z, et al. Destructive effect of percutaneous hydrochloric acid injection therapy for liver cancer–a preliminary experimental and clinical study. Gan To Kagaku Ryoho. 2006;33(12):1852–6.PubMedGoogle Scholar