Low expression of centrosomal protein 78 (CEP78) is associated with poor prognosis of colorectal cancer patients
- Meifang Zhang†1, 2,
- Tingmei Duan†1, 3,
- Li Wang1, 3,
- Jianjun Tang1, 3,
- Rongzhen Luo1, 2,
- Ruhua Zhang1, 3 and
- Tiebang Kang1, 3Email author
© The Author(s) 2016
Received: 24 July 2015
Accepted: 22 December 2015
Published: 29 June 2016
Centrosomal protein 78 (CEP78) has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains unclear. This study aimed to investigate the role and the clinical value of CEP78 in colorectal cancer (CRC).
Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to examine CEP78 expression in CRC tissues and adjacent noncancerous tissues. The association between CEP78 expression and clinical outcomes of CRC patients was determined. The effect of CEP78 on cell growth was examined in vitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, colony formation, and flow cytometry assays and in vivo using a nude mouse model.
The expression level of CEP78 was significantly lower in tumor tissues than in the adjacent normal tissues (P < 0.01). Low CEP78 expression was significantly associated with poor differentiation (P = 0.003), large tumor size (P = 0.017), lymphatic metastasis (P = 0.034), distant metastasis (P = 0.029), and advanced stage (P = 0.011). Kaplan–Meier analysis indicated that patients with low CEP78 expression had shorter survival than those with high CEP78 expression (P < 0.01). Overexpression of CEP78 in CRC cells significantly reduced cell viability and colony formation in vitro and halted tumor growth in vivo. Further study showed that CEP78 reintroduction in CRC cells resulted in G2/M phase arrest rather than cell apoptosis.
CEP78 might function as a tumor suppressor and serve as a novel prognostic marker in CRC.
Colorectal cancer (CRC) is the third most prevalent cancer worldwide, with over 1.4 million new cancer cases diagnosed and 693,900 deaths in 2012 . Additionally, the incidence and mortality of CRC are increasing yearly in China . The development of CRC is a long and complicated process accompanied by the combined activation of oncogenes and inactivation of tumor suppressor genes [3, 4]. Although an increasing number of molecules have been found to be implicated in CRC over the years, it is still necessary and urgent to identify genes that are crucial for tumor development in specific genetic contexts, which could further improve the early detection, prevention, intervention, and prognostic evaluation of patients with CRC.
The centrosome is a critical cellular organelle that functions as the microtubule-organizing center of cells and is critically involved in cell division [3, 5]. Centrosomal proteins (CEPs) are usually defined as the molecules that are localized at the centrosome and participate in the regulation of centrosome-related function . For instance, CEP76 specifically prevents centriole re-duplication by limiting duplication to one per cell cycle . CEP120 is required for centriole duplication, maturation, and subsequent ciliogenesis, which physiologically affects cerebellar and embryonic development . Regarding the cell cycle, centrosome duplication is periodically regulated in a highly choreographed manner and is accordingly coupled to activities of cyclin-dependent kinases (CDKs). Reciprocally, the activities of CDKs are affected by centrosome status. The inhibition or silencing of several centrosome-associated proteins, such as dynactin, poly(ADP-ribose) polymerase 3 (PARP3), centriolin, and A-kinase anchor protein 450 (AKAP450), could block cell cycle progression . Because dysregulation of the cell cycle commonly occurs during tumor development , CEPs potentially participate in tumorigenesis under certain genetic contexts. Recently, CEP55 was found to be overexpressed and positively correlated with tumor growth in CRC [11, 12]. However, the functions of CEPs in tumor development remain elusive and merit further investigation.
The CEP78 gene, located on chromosome 9q21.2, encodes a 78-kDa protein CEP78. CEP78 was identified as a component of the centrosome through mass spectrometry-based proteomic analysis. However, whether and how CEP78 is coordinated with centrosome activities remain unknown . A previous study demonstrated that CEP78 might be involved in treatment-associated immune responses in patients with prostate cancer . However, the clinical implication and functions of CEP78 relevant to tumorigenesis are largely unknown.
In this study, the expression of CEP78 in CRC was determined. Additionally, the relationship between the clinicopathologic parameters of CRC patients and CEP78 was examined. The antitumor effect of CEP78 was investigated in vitro and in vivo.
Cell lines and patient tissue samples
Association between clinicopathologic variables of colorectal cancer patients and centrosomal protein 78 (CEP78) expression
CEP78 expression [cases (%)]
≤ 50 years
> 50 years
Depth of tumor
T1 + T2
T3 + T4
I + II
III + IV
CRC cell lines (SW480, SW620, HCT116, HT29, DLD1, LOVO, RKO, and THC8307) were obtained from the American Type Culture Collection (ATCC) and cultured according to the instructions of ATCC.
Construction of stable cell lines overexpressing CEP78
Full-length human CEP78 cDNA was cloned into a pSin-puro vector (GenePharma, Shanghai, China), and CEP78 was verified by DNA sequencing. The primers were 5′-GGAATTCCATATGACCATGATCGACTCCGTGAAGCTG-3′ (forward) and 5′-CTAGCTAGCTCAGGAATGCAGGTCCTTTCC-3′ (reverse). pSin-puro-CEP78 or the pSin-puro empty vector was co-transfected with pMD.2G and psPAX2 into HEK-293T cells for 48 h. The recombinant viruses were collected and used to infect HT29 and HCT116 cells, which were cultured with 8 μg/mL polybrene for 24 h. Stable lines were selected with 1 μg/mL of puromycin for 2 weeks.
RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR)
The total RNA of fresh CRC specimens was isolated using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. First-strand cDNA was synthesized using PrimeScript® RT reagent kit with gDNA Eraser (Fermentas, Burlington, ON, Canada). Semi-qRT-PCR and qRT-PCR were performed for the detection of CEP78 mRNA using Advantage HD DNA Polymerase Mix (Takara, Shimogyo-ku, Kyoto, Japan) and SYBR® Premix Ex Taq™ II (Takara), respectively. The primer sequences were as follows: 5′-TGGCAGGGAGCAGATCACA-3′ (forward) and 5′-AAGCCAGCCATACAGTCAAGA-3′ (reverse) for CEP78; 5′-ACAGTCAGCCGCATCTTCTT-3′ (forward) and 5′-GACAAGCTTCCCGTTCTCAG-3′ (reverse) for GAPDH. The qRT-PCR reaction conditions were as follows: 98 °C for 2 min followed by 35 cycles of 98 °C for 10 s, 60 °C for 5 s, 72 °C for 30 s, and 72 °C for 10 min, and, finally, hold at 4 °C. The products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide for visualization using ultraviolet light.
Western blotting was performed as previously described . Cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes, which were then incubated with anti-CEP78 antibody (Sigma, St. Louis, MO, USA) in 5% non-fat milk. Then, the membrane was incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody. The blots were then visualized using an electrochemiluminescence (ECL) kit (Beyotime, Nangtong, Jiangsu, China).
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the cell viability as previously described . Cells were seeded at a density of 2500 or 3000 per well in 96-well microplates. The cells were incubated with MTT for 4 h, the optical density (OD) was detected at 490 nm with a microplate reader, and measurements were acquired once per day for 5 days. The results are presented as the mean ± standard error of mean (SEM) of three independent experiments.
Colony formation assay
Cells were plated in the 6-well culture plates at 250 cells per well, and each group had 3 wells. Cells were washed twice with phosphate-buffered saline (PBS) after incubation for 15 days at 37 °C, and then stained with Giemsa solution. The number of colonies containing ≥50 cells was counted under a light microscope (Olympus, Orinpasu Kabushiki-gaisha, Japan).
IHC was performed as previously described . Sections of paraffin-embedded specimens (4 μm in thickness) were baked and deparaffinized. Sections were stained with an anti-CEP78 antibody (Sigma Aldrich), and the antigen was detected using a secondary anti-rabbit HRP-conjugated antibody. Subsequently, the sections were stained with 3,3′diaminobenzidine (DAB) and counterstained with hematoxylin. CEP78 expression was evaluated by two independent pathologists. The IHC staining score was calculated. Using the H-score method, we multiplied the percentage score by the staining intensity score. The percentage of positively stained cells was scored as 0 (0%), 1 (1%–25%), 2 (26%–50 %), 3 (51%–75%), or 4 (76%–100%). Staining intensity was scored as 0 (negative staining), 1 (weak staining), 2 (moderate staining), or 3 (strong staining). The median score was chosen as the cut-off value to define low and high CEP78 expression .
The effect of CEP78 on apoptosis was examined. HT29 and HCT116 cells were transfected with a CEP78 overexpression vector for 48 h. Cells were then collected by centrifugation at 1000 r/min for 10 min and treated with Annexin V-FITC and propidium iodide (Roche, New York, NY, USA) for 15 min. The cell suspension was immediately analyzed by flow cytometry (Beckman-Coulter, Inc, Fullerton, CA, USA) to evaluate cell apoptosis. At least 10,000 events were collected for each sample.
Twenty-four male BALB/c athymic nude mice (4 weeks old) were obtained from Guangdong Medical Laboratory Animal Center (Guangzhou, China). Mice were randomly separated into the empty vector group and the CEP78 group with inoculation of HCT116 or HT29 cells. Each group included 6 mice. All animal experiments were performed in the animal institute of SYSUCC according to the principles and procedures approved by the Medical Experimental Animal Care Commission of SYSUCC. To assess CRC tumor growth in vivo, 1 × 106 HCT116 or HT29 cells were injected subcutaneously into the dorsal flank of each mouse. Tumor size was measured every 2 days. After 20 days, the mice were euthanized, and the xenografts were weighed.
SPSS software (version 16.0, Chicago, IL, USA) was used for statistical analysis. The relationship between CEP78 expression and the clinicopathologic parameters was examined by Student’s t test (Fisher’s exact test was chosen when the minimum expected cell count was less than 5). Overall survival (OS) curves were plotted by the Kaplan–Meier method and analyzed using the log-rank test. Univariate and multivariate Cox regression analyses were used to evaluate survival data. Differences were considered statistically significant when P values were less than 0.05 (two tailed).
The expression level of CEP78 was decreased in CRC tissues
Association between CEP78 expression and clinicopathologic factors
Clinicopathologic factors were further analyzed between the high and low CEP78 expression groups. As shown in Table 1, CRC patients with low CEP78 expression had a higher tendency to exhibit poor differentiation (P = 0.003), large tumor size (P = 0.017), lymphatic metastasis (P = 0.036), distant metastasis (P = 0.019), and advanced stage (P = 0.008). However, we did not find significant associations between CEP78 expression and other clinicopathologic parameters, such as age, gender, and depth of tumor (P > 0.05).
Low expression of CEP78 was associated with poor prognosis in CRC patients
Univariate and multivariate analyses of prognostic values of clinicopathologic features and CEP78 expression for overall survival of CRC patients
HR (95 % CI)
HR (95 % CI)
Age (≤50 years vs. >50 years)
Gender (female vs. male)
Tumor size (≤4 cm vs. >4 cm)
Tumor differentiation (well vs. moderate vs. poor)
Depth of tumor (T1 + T2 vs. T3 + T4)
Lymphatic metastasis (absent vs. present)
Distant metastasis (absent vs. present)
TNM stage (I + II vs. III + IV)
CEP78 expression (low vs. high)
Overexpression of CEP78 inhibited CRC proliferation in vitro
Overexpression of CEP78 impaired the growth of CRC in vivo
Centrosome amplification, a hallmark of cancer, accounts for the unlimited proliferation of human cancers. Centrosome duplication is strictly controlled by a series of checkpoints in which CEPs play essential roles . In this study, we showed that the expression of CEP78, a centrosome component, was markedly decreased in a large cohort of 237 patients with CRC. The silencing of CEP78 was closely associated with large tumor size, advanced tumor stage, lymphatic metastasis, and distant metastasis. Because patients with CRC often experience metastasis to the lymph nodes, liver, and lungs at late stages, CEP78 may serve as a predictor for tumor metastasis in CRC.
Patients with low CEP78 expression in our cohort were more likely to have shorter survival than those with high CEP78 expression. It has been well known that centrosome abnormality occurs in many types of cancer and is associated with poor outcomes . Although no evidence indicates that CEP78 plays a role in centrosome duplication, one of its homologous proteins, CEP76, has been demonstrated to regulate the centrosome reduplication. Enforced CEP76 expression specifically inhibits centriole amplification but not normal centriole duplication , indicating that CEPs are capable of limiting centrosome copies. Therefore, we assume that lack of CEP78 results in centrosome amplification, and subsequently contributes to poor outcomes in CRC. Our data suggest that CEP78 may be of clinical significance, not only due to the large sample size in our study but also because CRC lacks common prognostic biomarkers.
CEPs are differentially expressed in human cancers. For example, CEP55 was demonstrated to be highly expressed in colon carcinoma , oral cavity squamous cell carcinoma , and bladder transitional cell carcinoma . Reduction in CEP63 expression was found in bladder cancer . Our data revealed that CEP78 expression was decreased in CRC. In vitro data showed that CEP78 overexpression significantly reduced cell viability and colony formation of CRC HT29 and HCT116 cells. The anti-tumor effect of CEP78 was further supported because CEP78 overexpression halted the growth of CRC xenografts. Mechanistically, CEP78 overexpression efficiently inhibited cell growth via arresting CRC cells at the G2/M phase. This is consistent with the roles of other CEPs in cell cycle regulation. Slaats et al.  showed that knockdown of CEP164 expression led to S phase arrest in renal cells. Ruiz-Miró et al.  reported that overexpression of CEP57 hindered NIH-3T3 cells at S phase. In our study, cell apoptosis was not observed in CRC cells with CEP78 overexpression. Collectively, our data provide evidence that CEP78 controls cell proliferation partly by modulating G2/M phase transition.
In summary, our data show that the expression of CEP78 is remarkably decreased in patients with CRC. Furthermore, the expression of CEP78 is robustly related to unfavorable clinical outcomes in patients with CRC. CEP78 overexpression suppressed cell growth via inducing G2/M phase arrest. This study therefore suggests CEP78 as a potential prognostic and therapeutic biomarker for CRC.
MZ and TK contributed to conception and design of the study and drafted the manuscript; TD, LW, JT, RL, and RZ contributed to data analysis and interpretation; MZ and TD participated in data collection and literature research. All authors read and approved the final manuscript.
We thank Dr. Yuhui Jiang at MD Anderson Cancer Center and the members of Dr. Kang’s laboratory for their helpful comments on the manuscript.
The authors declare that they have no competing interests.
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- Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65(2):87–108.View ArticlePubMedGoogle Scholar
- Chen WQ, Zheng RS, Zhang SW, Zeng HM, Zou XN. The incidences and mortalities of major cancers in China, 2010. Chin J Cancer. 2014;33(8):402–5.PubMedPubMed CentralGoogle Scholar
- Network Cancer Genome Atlas. Comprehensive molecular characterization of human colon and rectal cancer. Nature. 2012;487(7407):330–7.View ArticleGoogle Scholar
- Jia Y, Guo M. Epigenetic changes in colorectal cancer. Chin J Cancer. 2013;32(1):21–30.View ArticlePubMedPubMed CentralGoogle Scholar
- Zyss D, Gergely F. Centrosome function in cancer: guilty or innocent? Trends Cell Biol. 2009;19(7):334–46.View ArticlePubMedGoogle Scholar
- Andersen JS, Wilkinson CJ, Mayor T, Mortensen P, Nigg EA, Mann M. Proteomic characterization of the human centrosome by protein correlation profiling. Nature. 2003;426(6966):570–4.View ArticlePubMedGoogle Scholar
- Tsang WY, Spektor A, Vijayakumar S, Bista BR, Li J, Sanchez I, et al. Cep76, a centrosomal protein that specifically restrains centriole reduplication. Dev Cell. 2009;16(5):649–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Wu C, Yang M, Li J, Wang C, Cao T, Tao K, et al. Talpid3-binding centrosomal protein Cep120 is required for centriole duplication and proliferation of cerebellar granule neuron progenitors. PLoS ONE. 2014;9(9):e107943.View ArticlePubMedPubMed CentralGoogle Scholar
- Srsen V, Merdes A. The centrosome and cell proliferation. Cell Div. 2006;1:26.View ArticlePubMedPubMed CentralGoogle Scholar
- Malumbres M, Barbacid M. Cell cycle, CDKs and cancer: a changing paradigm. Nat Rev Cancer. 2009;9(3):153–66.View ArticlePubMedGoogle Scholar
- Inoda S, Morita R, Hirohashi Y, Torigoe T, Asanuma H, Nakazawa E, et al. The feasibility of Cep55/c10orf3 derived peptide vaccine therapy for colorectal carcinoma. Exp Mol Pathol. 2011;90(1):55–60.View ArticlePubMedGoogle Scholar
- Sakai M, Shimokawa T, Kobayashi T, Matsushima S, Yamada Y, Nakamura Y, et al. Elevated expression of C10orf3 (chromosome 10 open reading frame 3) is involved in the growth of human colon tumor. Oncogene. 2006;25(3):480–6.PubMedGoogle Scholar
- Nesslinger NJ, Sahota RA, Stone B, Johnson K, Chima N, King C, et al. Standard treatments induce antigen-specific immune responses in prostate cancer. Clin Cancer Res. 2007;13(5):1493–502.View ArticlePubMedGoogle Scholar
- Sobin LHWC. TNM Classification of malignant tumours. 6th ed. New York: Wiley; 2002. p. 131–41.Google Scholar
- Xu S, Feng Z, Zhang M, Wu Y, Sang Y, Xu H, et al. hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene. 2011;30(19):2219–29.View ArticlePubMedGoogle Scholar
- Tang J, Wang G, Zhang M, Li FY, Sang Y, Wang B, et al. Paradoxical role of CBX8 in proliferation and metastasis of colorectal cancer. Oncotarget. 2014;5(21):10778–90.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang MF, Zhang ZY, Fu J, Yang YF, Yun JP. Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma. J Transl Med. 2009;7:110.View ArticlePubMedPubMed CentralGoogle Scholar
- Chan JY. A clinical overview of centrosome amplification in human cancers. Int J Biol Sci. 2011;7(8):1122–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen CH, Chien CY, Huang CC, Hwang CF, Chuang HC, Fang FM, et al. Expression of FLJ10540 is correlated with aggressiveness of oral cavity squamous cell carcinoma by stimulating cell migration and invasion through increased FOXM1 and MMP-2 activity. Oncogene. 2009;28(30):2723–37.View ArticlePubMedGoogle Scholar
- Singh PK, Srivastava AK, Rath SK, Dalela D, Goel MM, Bhatt ML. Expression and clinical significance of centrosomal protein 55 (CEP55) in human urinary bladder transitional cell carcinoma. Immunobiology. 2015;220(1):103–8.View ArticlePubMedGoogle Scholar
- Buim ME, Soares FA, Sarkis AS, Nagai MA. The transcripts of SFRP1, CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder. Oncology. 2005;69(6):445–54.View ArticlePubMedGoogle Scholar
- Slaats GG, Ghosh AK, Falke LL, Le Corre S, Shaltiel IA, van de Hoek G, et al. Nephronophthisis-associated CEP164 regulates cell cycle progression, apoptosis and epithelial-to-mesenchymal transition. PLoS Genet. 2014;10(10):e1004594.View ArticlePubMedPubMed CentralGoogle Scholar
- Ruiz-Miró M, Colomina N, Fernandez RM, Gari E, Gallego C, Aldea M. Translokin (Cep57) interacts with cyclin D1 and prevents its nuclear accumulation in quiescent fibroblasts. Traffic. 2011;12(5):549–62.View ArticlePubMedGoogle Scholar